Phenotype of NLCs, fresh blood monocytes, cultured cytokine-derived dendritic cells, or cultured cytokine-derived macrophages.

Blood mononuclear cells from patients with CLL were cultured for 14 days. In parallel, monocytes from nonleukemic donors were cultured with either GM-CSF and IL-4, or M-CSF and TNF-α to induce differentiation into dendritic cells or macrophages, respectively. Adherent cells were harvested and then stained with fluorochrome-conjugated isotype control mAbs or fluorochrome-conjugated mAbs specific for a cell surface antigen, as indicated to the left of each set of bars. The bars indicate the mean MFIR for NLCs (▪), freshly isolated blood monocytes (■), monocyte-derived dendritic cells (▨), or macrophages (░) from at least 3 different donors. The error bars indicate the SD about the mean MFIR. An asterisk indicates that the mean MFIR of one type of cells for a particular antigen is significantly different from that of NLCs.