CD14⁺ cells differentiate into NLCs in the presence of CLL B cells but not normal B cells.

CD14⁺ mononuclear cells were isolated from the blood of healthy donors and then cocultured with sorted CD19⁺ blood mononuclear cells from either patients with CLL (A) or healthy donors (B), or they were cultured alone (C). Fourteen days later, the adherent cells were examined for morphology after May-Giemsa staining.