American Society of Hematology

Fig. 5.

$Fig. 5. Size threshold for internalization of anti-PECAM/beads by REN/PECAM cells and HUVECs. / (A-D) Anti-PECAM/beads with effective diameters of 130 nm (A), 150 nm (B), 310 nm (C), or 560 nm (D) were incubated with REN/PECAM cells for 1 hour at 37°C, washed, fixed, and counterstained with Texas Red–conjugated goat antimouse IgG without permeabilization. Thus, surface-bound beads appear yellow, while internalized beads appear green. Bar, 10 μm. (E) The internalized and surface-bound conjugate fractions of sized anti-PECAM/beads were determined in triplicate by image analysis. The percentage of internalized 560-nm beads was significantly lower than for smaller bead preparations (*P < .05).$

Size threshold for internalization of anti-PECAM/beads by REN/PECAM cells and HUVECs.

(A-D) Anti-PECAM/beads with effective diameters of 130 nm (A), 150 nm (B), 310 nm (C), or 560 nm (D) were incubated with REN/PECAM cells for 1 hour at 37°C, washed, fixed, and counterstained with Texas Red–conjugated goat antimouse IgG without permeabilization. Thus, surface-bound beads appear yellow, while internalized beads appear green. Bar, 10 μm. (E) The internalized and surface-bound conjugate fractions of sized anti-PECAM/beads were determined in triplicate by image analysis. The percentage of internalized 560-nm beads was significantly lower than for smaller bead preparations (*P < .05).

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