Antigen-binding properties of anti-PECAM/avidin/DNA complex.

(A) Binding of ³²P-DNA/avidin complex, formed at various molar ratios, to plastic wells coated with biotinylated IgG. (B) Binding of anti-PECAM/avidin/³²P-DNA (triangles) and IgG/avidin/³²P-DNA (circles) complexes to plastic wells coated with recombinant PECAM (closed symbols) or albumin (open symbols). (C) Binding of anti-PECAM/avidin/³²P-DNA (hatched bars) and IgG/avidin/³²P-DNA (closed bars) complexes to wells with PECAM-expressing cells (REN/PECAMs and HUVECs) or control cells (REN). The data are shown as amount of ³²P-DNA bound per well, mean ± SD, n = 3.