Fig. 1.
Validation of in situ methods.
(A) In situ hybridization with 35S-labeled antisense probes results in an accumulation of black silver grains, indicating RAG expression in cortical but not in medullary thymocytes of a human thymus. (B) Hybridization with sense control probes shows no specific labeling. (C) In situ hybridization reveals scattered RAG-positive cells in bone marrow, whereas (D) hybridization with sense probes shows no specific labeling.
