Fig. 1.
![Fig. 1. PNU156804 disrupts cell growth of activated, but not of unactivated, human T cells in a dose-dependent manner. / Proliferation of quiescent PHA-activated human T cells (5.0 × 104 cells/well) in the presence of 1 nM IL-2 was examined after treatment with increasing concentrations of PNU156804 (●) or inactive control PNU159744 (♦) for 16 hours at 37°C. Conversely, Jurkat cells were treated in an identical fashion with PNU156804 (▴). All cells were then pulsed with [3H]-thymidine (0.5 μCi [0.0185 MBq]/200 μL) for 4 hours, incorporated into a radiolabeled probe, and plotted on the abscissa expressed as total cpm (n = 6).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/2/10.1182_blood.v99.2.680/6/h80222002001.jpeg?Expires=1735847809&Signature=qy~wGHcNPGcGV-SuTJw0EvRheiZA7sm-rmbVRHSviZjIKON4nKBkd4FrgOJB4XDQm-KXoZMEDvjAaVs5TtKPNDSSsiSfa90AlNuoyKN~L2BWG3MCPoYHsaqqxhf6xKb2tgpFHviKwOqMTHNW4RveYJgjBFRcEqjVnfn44opItKxWYtuQLRo4Dt2apkG9dOXMw6469XBS0lF227PEKS5~hHC22HZ6lS3g~ehVondDPe5uvgnuQArGO~RomWP8zbws1zWOynifMNyX7In00Z7NMPueusrNSWEYPPxdeCpk7gexNGdJt4xd~qS5rPP5aVETu0TxHt58eQxTCXELuDi4Dw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PNU156804 disrupts cell growth of activated, but not of unactivated, human T cells in a dose-dependent manner.
Proliferation of quiescent PHA-activated human T cells (5.0 × 104 cells/well) in the presence of 1 nM IL-2 was examined after treatment with increasing concentrations of PNU156804 (●) or inactive control PNU159744 (♦) for 16 hours at 37°C. Conversely, Jurkat cells were treated in an identical fashion with PNU156804 (▴). All cells were then pulsed with [3H]-thymidine (0.5 μCi [0.0185 MBq]/200 μL) for 4 hours, incorporated into a radiolabeled probe, and plotted on the abscissa expressed as total cpm (n = 6).
