![Fig. 1. RBC membrane proteins, genomic DNA, and Jk transcript from the Jknull and control Jk(a+b+) donors. / (A) Immunoblot analysis. RBC membrane proteins (40 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted with antibodies against the N-terminal or C-terminal of the Jk/hUT-B1 protein.9 (B) Southern blot analysis: Genomic DNA (15 μg/lane) was digested with SacI (10 U/μg), resolved by agarose gel electrophoresis, transferred to a nylon membrane, and hybridized with a [32P]-labeled cDNA probe (exons 4 to 11).2 Size of DNA markers (kb) and exons in each fragment are given in the left and right margins, respectively. (C) Partial sequence analysis. Jknull transcript sequence from Alf-Express DNA sequencer (Amersham Pharmacia, Uppsala, Sweden). E*, intronic sequence found in the Jk(Δ4,5) transcript from the propositus but not in the Jk(a+b+) control. Genomic sequences were deposited to the European Molecular Biology Laboratory (EMBL) database under the accession numbers AJ316564 and AJ316565.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/3/10.1182_blood.v99.3.1079/5/h80322087001.jpeg?Expires=1735843812&Signature=VaWz2bYJciLhlJM6fHE9NGqoUd3hBTDUUF32b4Rn0plmDRdarL9P91lk0v6ie5kgeTTd1t~Ae~fTj6rvUINOFGsm9k78ns0pnYPhZTH5NIYR5qxr-YBPogM3kKUCQ-Kd1oZnrA7a8rMCt7JG7nPQQjy6MRdLXBUdSLb8GO7npY5ol~D8nq-wQt6vibttsW0pm8fafcmUihD7Lf1r8iLULzHHYU-wdB6NK~axT52cdVH~IGbJBExqxjr7Di0~ZAgc9MS0LVfmRTqtOYlu4gg2J~aUQZROm2iRDCSuY6wNAuKZ3dPQgDPr-T5LKu085p4e7c7FKFZWjqA2Oh9jVM4cnQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
RBC membrane proteins, genomic DNA, and Jk transcript from the Jknull and control Jk(a+b+) donors.
(A) Immunoblot analysis. RBC membrane proteins (40 μg) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted with antibodies against the N-terminal or C-terminal of the Jk/hUT-B1 protein.9 (B) Southern blot analysis: Genomic DNA (15 μg/lane) was digested with SacI (10 U/μg), resolved by agarose gel electrophoresis, transferred to a nylon membrane, and hybridized with a [32P]-labeled cDNA probe (exons 4 to 11).2 Size of DNA markers (kb) and exons in each fragment are given in the left and right margins, respectively. (C) Partial sequence analysis. Jknull transcript sequence from Alf-Express DNA sequencer (Amersham Pharmacia, Uppsala, Sweden). E*, intronic sequence found in the Jk(Δ4,5) transcript from the propositus but not in the Jk(a+b+) control. Genomic sequences were deposited to the European Molecular Biology Laboratory (EMBL) database under the accession numbers AJ316564 and AJ316565.
