Cellular localization of EGFP-LANA.

(A) Nuclear localization of EGFP-LANA in stably transfected 293 cells. Fluorescent image was superimposed on brightfield image using Kodak Microscopy Documentation System and Adobe Photoshop, version 5.5. (B) Fluorescent image superimposed on brightfield image demonstrates nuclear localization of EGFP-LANA in R1T cells. (C) Cytoplasmic localization and nuclear exclusion of EGFP-LANAΔN440 in R1T cells. (D) Same as panel D, but in 293 cells. (All images taken with a Nikon TS100F phase contrast microscope at × original 400 magnification).