Fig. 4.
Fig. 4. LANA transactivates the IL-6 promoter in R1T and 293 cells. / (A) Reporter gene expression in R1T cells transiently transfected with pIL6-1200/SEAP and EGFP-LANA or EGFP blank vectors. Results are the means of 3 experiments. (B) Transient transfection of pIL6-1200/SEAP with EGFP-LANA, EGFP-LANAΔN440, or EGFP blank in R1T cells. Experiments performed in duplicate. (C) Western blot for EGFP-LANA on protein extracts from 293-TetOff-EGFP-LANA cells were exposed to various concentrations of doxycycline. Nuclear protein (20 μg) was loaded in each lane and hybridized with an anti-EGFP antibody (Clontech). Coomassie blue staining of gel demonstrated equal loading of protein in each lane. (D) pIL6-1200/SEAP was transiently transfected into 293-TetOff-EGFP LANA cells exposed to various concentrations of doxycycline. Results are means of 2 experiments.

LANA transactivates the IL-6 promoter in R1T and 293 cells.

(A) Reporter gene expression in R1T cells transiently transfected with pIL6-1200/SEAP and EGFP-LANA or EGFP blank vectors. Results are the means of 3 experiments. (B) Transient transfection of pIL6-1200/SEAP with EGFP-LANA, EGFP-LANAΔN440, or EGFP blank in R1T cells. Experiments performed in duplicate. (C) Western blot for EGFP-LANA on protein extracts from 293-TetOff-EGFP-LANA cells were exposed to various concentrations of doxycycline. Nuclear protein (20 μg) was loaded in each lane and hybridized with an anti-EGFP antibody (Clontech). Coomassie blue staining of gel demonstrated equal loading of protein in each lane. (D) pIL6-1200/SEAP was transiently transfected into 293-TetOff-EGFP LANA cells exposed to various concentrations of doxycycline. Results are means of 2 experiments.

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