Results after RT-PCR amplification with G.257/G.1225 primer pair screening all HLA-G isoforms.

Results are shown in (A) lesional and nonlesional skin and in (B) PBMCs of CL patients. Due to the low sensitivity of ethidium bromide staining, for some samples the bands are not visible. Using a melting curve analysis (data not shown) and the primer pair amplifying HLA-G1, it was possible to confirm that all the cases were transcribing HLA-G. The cDNAs from JEG-3 cell line and first trimester placenta (Pla) were used as positive controls. Instead of cDNA template, water (–) was run as a negative control; hs indicates healthy skin; l, lesional skin; n, nonlesional skin; BC, buffy coat preparation; and MWM, molecular weight marker.