Relative HLA-G1 transcriptional levels in primary CLs.

Values are expressed as mean ± SD GAPDH-balanced ratios (as a result of triplicate runs) multiplied by 10⁴. (A) Pattern of HLA-G1 expression in lesional (n = 17) versus nonlesional (n = 7) and a control healthy skin. The Student t test was performed; t = 2.54, P = .022. (B) Relative HLA-G1 transcriptional levels grouped according to the immunoreactivity with 4H84 mAb. Levels of HLA-G1 mRNA expression in cases showing no 4H84 immunoreactivity (n = 7) versus single-cell positivity (n = 5) versus HLA-G protein–positive lymphoma cases (n = 4). In some cases HLA-G immunoreactivity coincided with IL-10 expression (▾). NL indicates nonlesional skin.