Fig. 7.
Fig. 7. Leu278 at position −3 in the first SAP binding motif of CD150 partially stabilizes non–phosphotyrosine binding but is not required for phosphotyrosine-dependent interaction. / (A) Yeast 2-hybrid β-galactosidase assay for SAP/CD150 non–tyrosine phosphorylation–dependent interaction with CD150 or CD150 Lys276Asn or CD150 Leu278Ile. ▪, +SAP; ■, +pGBT9. (B) Altered yeast 2-hybrid using a modified Fyn (Fyn2YF R-Q, see “Materials and methods” for details) to phosphorylate CD150. β-Galactosidase assay to measure strength of the SAP/CD150 interaction data are representative of 3 separate experiments. ▪, +SAP + Gyn2YF R-Q; ■, +pBridge.

Leu278 at position −3 in the first SAP binding motif of CD150 partially stabilizes non–phosphotyrosine binding but is not required for phosphotyrosine-dependent interaction.

(A) Yeast 2-hybrid β-galactosidase assay for SAP/CD150 non–tyrosine phosphorylation–dependent interaction with CD150 or CD150 Lys276Asn or CD150 Leu278Ile. ▪, +SAP; ■, +pGBT9. (B) Altered yeast 2-hybrid using a modified Fyn (Fyn2YF R-Q, see “Materials and methods” for details) to phosphorylate CD150. β-Galactosidase assay to measure strength of the SAP/CD150 interaction data are representative of 3 separate experiments. ▪, +SAP + Gyn2YF R-Q; ■, +pBridge.

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