CD150 displays lateral mobility and spontaneously segregates into intercellular contacts with SAP.

Jurkat-Tag cells were transiently transfected with CD150-EGFP. After 24 hours the cells were fixed in methanol and stained with anti–SAP-Cy3 or Hoescht or both to visualize the nuclei. (A) Jurkat-Tag transfected with CD150-EGFP, counterstained with Hoescht. Arrow points to cell-cell contact rich in CD150. (B) Jurkat-Tag untransfected. (C) SAP colocalizes at the cell interface with CD150. Jurkat-Tag transfected with CD150-EGFP, stained with anti–SAP-Cy3 and Hoescht. Arrow points to cell interface. (D) Jurkat-Tag untransfected, stained with anti–SAP-Cy3. In the absence of CD150, SAP is expressed homogeneously throughout the cytoplasm.