Segregation of CD150 into cell-cell contacts is not dependent on SAP binding.

(A) Jurkat T cells stably transfected with SV40 large T antigen (Jurkat-TAg) were transiently transfected with CD150 or the 2 non–SAP-binding mutants Tyr13Phe or Thr13Ala. Expression levels were consistently 30% to 45%. (B) SAP binds to CD150, but not Tyr13Phe or Thr13Ala mutants in Jurkat TAg cells. (C) CD150 is concentrated on the plasma membrane at the cell-cell contact of adjacent Jurkat-TAg cells transfected with CD150 or Thr>Ala/Tyr>Phe mutants.