Depletion of fibrinogen and solid clot formation during TF-initiated clotting of whole blood.

Samples for fibrinogen and solid-clot analyses were obtained from experiments shown in Figure 5. Upper lanes in each panel represent soluble fibrinogen; lower parts represent solubilized solid clots. Panel A shows normal blood without factor VIIa addition; panel B, congenital hemophilia A blood without factor VIIa addition; and panel, C congenital hemophilia A blood with 10 nM factor VIIa addition. Arrows indicate clotting times. Fbg indicates fibrinogen; Fn, fibrin.