Enhanced leukocyte adhesion in blood vessels of TSP-2–deficient mice during skin inflammation.

Twenty-four hours after oxazolone challenge, mice were perfused with the lectin L esculentum to visualize leukocytes adherent to the luminal surface of blood vessels. Although only sparse leukocytes were found to be adherent to blood vessels of wild-type mice (A), an increased density of adherent leukocytes was detected in TSP-2–deficient mice (B). Scale bars = 100 μm. (C) Quantitative analyses of lectin-perfused skin revealed a 2.8-fold increase in leukocyte adhesion density in TSP-2–deficient mice (▪), as compared with wild-type mice (■). Data are expressed as mean ± SD. ***P < .001.