American Society of Hematology

Fig. 2.

$Fig. 2. B220lo. / c-kit+CD19− progenitors give rise to T and NK cells but fail to generate B and myeloid progeny in vitro. (A) B220+CD19+ (fraction a) FL cells isolated from wild-type embryos and B220loc-kit+CD19− cells (fraction e) isolated from wild-type, nu/+, andnu/nu were set under limiting dilution conditions in FTOC (upper panels) and with S17 stromal cells, KL, and IL-7 (lower panels). Plots of the percentage of negative wells per cell concentration and calculated frequencies are shown. (B) Total FL cells (■) and B220loc-kit+CD19− cells (♦) from wild-type C57BL/6 mice were cultured in methylcellulose, and erythromyeloid colonies were counted on day 7. (C) The same populations as in (B) were cultured on OP9 stromal cells in medium supplemented with IL-7, KL, and IL-2 for B, myeloid, and NK cell development. Cells were stained and analyzed by flow cytometry after 10 days of culture.$

B220^lo

c-kit⁺CD19⁻ progenitors give rise to T and NK cells but fail to generate B and myeloid progeny in vitro. (A) B220⁺CD19⁺ (fraction a) FL cells isolated from wild-type embryos and B220^loc-kit⁺CD19⁻ cells (fraction e) isolated from wild-type, nu/⁺, andnu/nu were set under limiting dilution conditions in FTOC (upper panels) and with S17 stromal cells, KL, and IL-7 (lower panels). Plots of the percentage of negative wells per cell concentration and calculated frequencies are shown. (B) Total FL cells (■) and B220^loc-kit⁺CD19⁻ cells (♦) from wild-type C57BL/6 mice were cultured in methylcellulose, and erythromyeloid colonies were counted on day 7. (C) The same populations as in (B) were cultured on OP9 stromal cells in medium supplemented with IL-7, KL, and IL-2 for B, myeloid, and NK cell development. Cells were stained and analyzed by flow cytometry after 10 days of culture.

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