Fig. 3.
In vitro generation of T and NK cells from single B220lo
c-kit+CD19− progenitors.Sorted cells were seeded at one cell per well in Terasaki plates. Wells were individually checked under a microscope, and wells containing a single cell were identified. Single cells were then placed in a hanging drop with one irradiated thymic lobe from Ly5 congenic embryos. Flow cytometric analysis of individual lobes was done at day 16 of FTOC.
