Dominant-negative c-Myb suppresses

RAG-2 promoter activity. (A) Effect of dominant-negative c-Myb on RAG-2 promoter activity in 293T cells. A quantity of 5 μg luciferase construct linked to the −86/+147 promoter region was transfected into 293T cells with or without 1 μg Pax-5 expression vector and 0.2 μg c-Myb expression vector, along with various amounts (0.013 μg, 0.32 μg, 8 μg) of pAct-ΔTA. Forty-eight hours later, luciferase activities were determined as in Figure 4. Error bars indicate deviation of 3 experiments. (B) Effect of dominant-negative c-Myb on RAG-2promoter activity in 18.8.1 cells. A quantity of 10 μg luciferase construct linked to −86/+147 promoter region was transfected into 18.8.1 cells together with various amounts (0.013 μg, 0.32 μg, and 8 μg) of pAct-ΔTA. Luciferase construct without promoter (None) was transfected as a control. Forty-eight hours later, luciferase activities were assayed as in Figure 1. Error bars indicate deviation of 3 experiments.