Fig. 4.
Protein levels of Bcl-2 family members in sensitive and resistant cells.
Protein levels were detected by Western blotting as described in “Materials and methods.” The antiapoptotic Bcl-2 family members, Bcl-2 and Bcl-xL, and the proapoptotic members, Bax, Bak, Bid, and Bad, were investigated because of their relevance to the process of cytochrome c release. Equal loading is evident from the Bcl-2, Bcl-xL, Bak, and Bid blots. Protein levels for Bcl-2, Bcl-xL, Bax, Bak, and Bid were also measured in a cell line developed with resistance to FaraA (CEM FaraA1000) but displaying cross-resistance to CdA. Data shown are representative of that obtained in 3 independent experiments.
