Combined developmental profiles of human ε, human γ, or galago γ transgenes driven by HS2 and HS3.

Panels A and B represent expression of the human ε (A) and galago γ (B) transgenes in HS2-ε-^galγ constructs (this study) and HS3-ε-^galγ constructs.3 For all these lines, the human ε and galago γ genes were expressed highly in embryonic life and were silenced in fetal life. Thus, in panels A and B, transgene expression levels in embryonic life is set to 100% for each line, and the level seen in fetal life is expressed relative to the embryonic level for that line. Panels C and D represent expression of the human ε (C) and human γ (D) transgenes in HS2-ε-^humγ constructs3 and in HS3-ε-^humγ constructs.4 For the ε gene, embryonic levels for each line are set at 100%, and fetal levels are normalized to this level as for panels A and B. For the human γ gene, however, expression was consistently highest in the fetal liver. Thus, the fetal expression level was set at 100%, and the embryonic level in each line was normalized to this level. Although human γ gene expression is variable in embryonic life, the difference in expression profile between the human γ gene and the galago γ gene is strikingly consistent.