Processing of components obtained from SCT and control subjects.

Study schema for processing and cryopreservation of PBSC products collected from 8 SCT donors and 8 control subjects. PBSC products from each donor were split. Half of each product was centrifuged to remove plasma, and the other half was treated for red blood cell removal, either by hydroxyethyl starch (HES) sedimentation (3 SCT and 4 control subjects) or ammonium chloride (ACK) lysis (5 SCT and 4 control subjects). Red blood cell depletion in SCT products could not be accomplished by HES sedimentation. The plasma or red cell–depleted products were again divided, and each half was cryopreserved in either a conventional 10% DMSO solution or in a solution composed of 5% DMSO/6% pentastarch/4% human serum albumin.