Effect of IgE receptor cross-linking and cytokines on mast cell adhesion to ECM proteins.

(A) Mast cells were precultured with SCF before the adhesion assay was performed in the presence of mAb 29C6, inducing IgE receptor cross-linking, SCF, IL-3, or IL-4. Results represent mean ± SD of 4 experiments. *P < .05 for significant difference (2-tailed paired t test) compared to the PBS control. (B) Mast cell adhesion to fibronectin in the presence of NGF, IFN-γ, TGF-β, TNF-α, IL-6, IL-8, IL-10, IL-13, C3a, C5a, SCF, and SCF together with IL-4. Results represent mean ± SD of 3 experiments. *P < .05 for significant difference (2-tailed paired t test) compared to the PBS control. (C) Time course of mast cell adhesion to fibronectin in response to SCF or IL-4. Mast cells were precultured with SCF. Mast cell adhesion was determined in the presence of SCF, IL-4, or buffer control, respectively, after 90, 180, and 360 minutes. Results represent mean ± SD of 3 experiments.