Western blot analysis of Bcl-2, Bcl-XL, and STAT5 expression in 32D/TREx/Ik6 clone 11 growing in IL-3–deprived medium with or without tetracycline.

Only Bcl-XL was up-regulated. Other Bcl-2 family proteins (Bak, Bax, and Mcl-1) were unaffected by Ik6 induction (data not shown). Bad, another Bcl-2 family protein, could not be detected in the 32D cell line. On indicates with tetracycline; off, without tetracycline.