Western blot analysis of Ik2, Ik6, Bcl-2, Bcl-XL, and STAT5 expression in a Cos-7/pRC/CMV/Ik2/TREx/Ik6 clone.

Expression was tested before (lane 2) and at 12 hours (lane 3), 16 hours (lane 4), and 24 hours (lane 5) after Ik6 induction. Lane 1 (Cos-7/TREx/empty) served as the negative control for Ik2 and 6, whereas lane 6 (Cos-7/pRC/CMV/Ik6) was the positive control for Ik6. Bcl-XL was up-regulated after induction of Ik6 (lanes 3, 4, and 5).