Fig. 4.
Heat shock induces formation of 5-LO products.
Freshly isolated PMNLs (5 × 106 in 1 mL PGC buffer) were preincubated at 45°C for the indicated times. Then, samples were brought to 37°C and 40 μM AA was added. After another 5 minutes at 37°C, 5-LO products were determined by HPLC. Results are given as mean + SE (n = 3-5). Student t test; *P < .05; **P < .01.
