Determination of EBV-specific cell frequencies in PBMCs, Co cells, and GMCs by LDAs.

The pCTL (left panels) and pTh (right panels) frequencies as determined by LDAs in PBMCs (A-B), Co cells (C-D), and GMCs (E-F). The indicated responder cells were cultured in the absence (closed circles) or the presence of irradiated autologous (open circles) or allogeneic (closed triangles) B-EBV cell lines during the sensitization phase. For pCTL determination, the target cells were the autologous (circles) or allogeneic (triangles) cell lines used for sensitization. The frequencies are indicated for each culture, in front of their respective regression curve. Data are from one experiment representative of those shown in Figure 2. ND indicates not detectable.