SDF-1 decreases the expression of APO2.7 apoptosis-associated mitochondrial protein in PB Inc⁺CD34⁺ cells.

PB CD34⁺ cells freshly purified after incubation on a plastic support (Inc⁺) were incubated (1 × 10⁵ cells/mL) under apoptosis-inducing culture conditions (serum- and cytokine-free medium [IMDM]). Cells harvested after a 6-hour incubation program were processed for apoptosis assessment by using the mitochondrial APO2.7 antigen detection assay as indicated in “Materials and methods.” The biologic effect of SDF-1 on apoptosis was evaluated by adding 0.5 ng/mL rhSDF-1α to the culture medium. Biologic specificity of SDF-1 was demonstrated by using an anti–SDF-1 antibody (5 ng/mL) or its isotype control (5 ng/mL). A histogram from a typical donor is presented. The MFI (AU) of cells positive for APO2.7 is shown for each condition.