![Fig. 5. SDF-1 modulates apoptosis-regulatory gene products in PB Inc+CD34+ cells. / PB CD34+ cells freshly purified after incubation on a plastic support (Inc+) were incubated (1 × 105 cells/mL) under apoptosis-inducing culture conditions (serum- and cytokine-free medium [IMDM]). Freshly isolated cells or cells harvested after a 72-hour incubation program were processed for intracellular detection of (A) antiapoptotic regulatory proteins (Bcl-2 and Bcl-xL) and (B) proapoptotic regulatory proteins (Bad and Bax) as indicated in “Materials and methods.” Modulation of Bcl-2 homolog protein expressions was evaluated in the absence or presence of SDF-1 (0.05 ng/mL). Data from at least 3 different donors with similar results were analyzed. Histograms from a typical donor are presented. The percentage or MFI (AU) or both of positive cells for Bcl-2, Bcl-xL, Bad, and Bax are shown within the histograms. The left-hand histogram represents the negative control (IgG).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/4/10.1182_blood.v99.4.1117/6/h80422156005.jpeg?Expires=1735818646&Signature=UccHd8M4NoHezBoIg8Ct8W~0FPCoGZ1nAhjfrGNaINq1hUCUwcsHpgqYZA~-mh0pvyz7BuooTf58D2XUvm76O71nFr0S7OMCAyhoZZ1woDQoX-WDV5qNw24RKZkmkc0z1SLxUV3rr99soC1q5PYCtNhRAd8DxiwUWaA0poanpKA6MurJGMl4ii8ogiglh5GdfuJIEbisBGGvB0dkwxhgB934ncI9vX1lmWUbo8L7p1fFz7AG7hfmo5d8kXkPdivit8c~n9DRzZoRgW-5YyfFPJU3K4LCMov0yo1ocdFeYjIPFPVO-Qhz1MYWlu9EytmU6twynG4V1U7d0kpSOkBJWA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SDF-1 modulates apoptosis-regulatory gene products in PB Inc+CD34+ cells.
PB CD34+ cells freshly purified after incubation on a plastic support (Inc+) were incubated (1 × 105 cells/mL) under apoptosis-inducing culture conditions (serum- and cytokine-free medium [IMDM]). Freshly isolated cells or cells harvested after a 72-hour incubation program were processed for intracellular detection of (A) antiapoptotic regulatory proteins (Bcl-2 and Bcl-xL) and (B) proapoptotic regulatory proteins (Bad and Bax) as indicated in “Materials and methods.” Modulation of Bcl-2 homolog protein expressions was evaluated in the absence or presence of SDF-1 (0.05 ng/mL). Data from at least 3 different donors with similar results were analyzed. Histograms from a typical donor are presented. The percentage or MFI (AU) or both of positive cells for Bcl-2, Bcl-xL, Bad, and Bax are shown within the histograms. The left-hand histogram represents the negative control (IgG).
