PB Inc⁻ CD34⁺CD38⁺cells express intracellular SDF-1.

Representative flow cytometry profiles for Inc⁻ and Inc⁺ cells (1 × 10⁵ cells/mL) isolated from freshly purified PB CD34⁺ cells (A) and apoptosis-induced cells (B) after staining for CD34 and CD38 antigens and intracellular SDF-1 as indicated in “Materials and methods.” The logarithm fluorescence of intracellular SDF-1 is expressed in channel numbers. The shaded histogram shows results from staining with irrelevant isotype-matched control antibody, the open histogram, results from staining with SDF-1 mAb.