American Society of Hematology

Fig. 10.

$Fig. 10. SDF-1 triggers G0 PB Inc−CD34+ cells into cycle. / PB CD34+ cells purified immediately after density gradient separation (Inc−) were incubated (1 × 105cells/mL) in a serum- and cytokine-free Stemα-A medium in the presence or absence of SDF-1 (0.01 ng/mL to 0.5 ng/mL). Freshly isolated cells (A) and cells harvested after a 48-hour incubation (B) were processed for cell cycle fractionation by using simultaneous staining for DNA content (PI) and for Ki67 expression as indicated in “Materials and methods.” This assay allowed discrimination of G0 from G1 and S+G2/M cells as shown in each histogram. Arbitrary quadrants were drawn on the basis of isotype-matched negative control profiles (first dot plot of each panel). The results shown are for one experiment representative of the 3 to 4 performed.$

SDF-1 triggers G₀ PB Inc⁻CD34⁺ cells into cycle.

PB CD34⁺ cells purified immediately after density gradient separation (Inc⁻) were incubated (1 × 10⁵cells/mL) in a serum- and cytokine-free Stemα-A medium in the presence or absence of SDF-1 (0.01 ng/mL to 0.5 ng/mL). Freshly isolated cells (A) and cells harvested after a 48-hour incubation (B) were processed for cell cycle fractionation by using simultaneous staining for DNA content (PI) and for Ki67 expression as indicated in “Materials and methods.” This assay allowed discrimination of G₀ from G₁ and S+G₂/M cells as shown in each histogram. Arbitrary quadrants were drawn on the basis of isotype-matched negative control profiles (first dot plot of each panel). The results shown are for one experiment representative of the 3 to 4 performed.

This Feature Is Available To Subscribers Only