American Society of Hematology

Fig. 11.

$Fig. 11. SDF-1 induces PB Inc+ CD34+cell cycle progression from G1 to S+G2/M. / PB CD34+ cells purified after incubation on a plastic support (Inc+) were incubated (1 × 105cells/mL) in a serum- and cytokine-free Stemα-A medium in presence or absence of SDF-1 (0.01 ng/mL to 0.5 ng/mL). Freshly isolated cells (A) and cells harvested after a 48-hour incubation (B) were processed for cell cycle fractionation by using simultaneous staining for DNA content (PI) and for Ki67 expression as indicated in “Materials and methods.” Arbitrary quadrants were drawn on the basis of isotype-matched negative control profiles (first dot plot of each panel). Maximal effect of SDF-1 was obtained for 0.05 ng/mL. Cell cycle fractionation percentages shown in each histogram were obtained from one experiment representative of the 3 to 4 performed.$

SDF-1 induces PB Inc⁺ CD34⁺cell cycle progression from G₁ to S+G₂/M.

PB CD34⁺ cells purified after incubation on a plastic support (Inc⁺) were incubated (1 × 10⁵cells/mL) in a serum- and cytokine-free Stemα-A medium in presence or absence of SDF-1 (0.01 ng/mL to 0.5 ng/mL). Freshly isolated cells (A) and cells harvested after a 48-hour incubation (B) were processed for cell cycle fractionation by using simultaneous staining for DNA content (PI) and for Ki67 expression as indicated in “Materials and methods.” Arbitrary quadrants were drawn on the basis of isotype-matched negative control profiles (first dot plot of each panel). Maximal effect of SDF-1 was obtained for 0.05 ng/mL. Cell cycle fractionation percentages shown in each histogram were obtained from one experiment representative of the 3 to 4 performed.

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