Fig. 13.
Western blot analysis of cyclin D1 in PB Inc− CD34+ cells.
PB CD34+ cells immediately purified after density gradient separation (Inc−) were incubated (1 × 105cells/mL) in a serum- and cytokine-free Stemα-A medium in the presence or absence of SDF-1 (0.5 ng/mL). After a 48-hour incubation, cells were harvested and processed for Western blot analysis of cyclin D1 as indicated in “Materials and methods.” Biologic specificity of SDF-1 was demonstrated by using an anti–SDF-1 antibody (5 ng/mL). Results from a typical donor are presented.