Fig. 6.
![Fig. 6. Pulse chase analysis of β3 integrin biosynthesis. / Wild-type, His280Pro, or Arg216Gln/Leu292Serβ3 cDNA was cotransfected into 293 cells with either (A) wild-type αIIb cDNA or (B) wild-type αv cDNA. Cells were labeled one day after transfection with 0.4 mCi/mL (14.8 MBq) [35S]methionine for 30 minutes and chased with media containing 50 μg/mL of nonradioactive methionine for various periods of time as indicated. Immunoprecipitation was performed using either (A) TP80 or (B) LM142. Precipitates were separated by 6% SDS-PAGE under reducing conditions. Results are representative of 3 separate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/3/10.1182_blood.v99.3.931/5/h80322098006.jpeg?Expires=1735495592&Signature=vieN8B8M~~tiN8PeVl-P~XUlIYHfNdJlmxxi-DDN9QrTOgwUFS1i6fZsfQmCWJ7p2gaJGdFskyJKn6sUDhEsR~MQ8jvW9SQT4RiddWfmdeHcFaMFpvFxk51zRc9WZR8A9Zatdv0VhLvtzNDrpJFYiiALQCsZoyLaQ~BXsaLNYnJj-KBZ8H4BrNRk8s2AfBvQl5rpeRYQfrtlow-ycDAoL2HEecZ4hGloHULIp0XzqTLX2aBwZ-64ohq-A3UoPOt2YmJ44Nk65Ehy9Q47dvW3bTbTZk61LpQnEurkEqjCLQCyE2MMUYSYBb0USmOxBB4VYKFJL0-VvH7X7-Igg5bGdw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse chase analysis of β3 integrin biosynthesis.
Wild-type, His280Pro, or Arg216Gln/Leu292Serβ3 cDNA was cotransfected into 293 cells with either (A) wild-type αIIb cDNA or (B) wild-type αv cDNA. Cells were labeled one day after transfection with 0.4 mCi/mL (14.8 MBq) [35S]methionine for 30 minutes and chased with media containing 50 μg/mL of nonradioactive methionine for various periods of time as indicated. Immunoprecipitation was performed using either (A) TP80 or (B) LM142. Precipitates were separated by 6% SDS-PAGE under reducing conditions. Results are representative of 3 separate experiments.
