Ability of CD4⁺ and CD8⁺ T-cell subsets from PB and BM to induce histopathologic lesions of GVH disease in the colon and skin.

Sections are stained with hematoxylin and eosin and the original magnification is × 400. Each panel is representative of 4 recipients. (A,B) Intestine and skin of a BALB/c recipient with severe clinical GVH disease injected 50 days earlier with TCD BM and sorted PB CD4⁺ T cells. There is an expanded lamina propria with marked lymphocytic infiltration (asterisk) and infiltration into the glandular epithelium (black arrow). There are apoptotic bodies in the glandular epithelium also (white arrow). The skin appears to be normal. (C,D) The colon and skin sections of a healthy recipient injected 45 days earlier with TCD BM and BM CD4⁺ T cells. Plump mucin-containing glandular cells are seen lining the crypts with little or no inflammation. The skin appears to be normal. (E,F) The tissue sections of a recipient with slight diarrhea injected 45 days earlier with BM NK1.1⁻ CD4⁺ T cells. The colon shows lesions of GVH disease, but the skin appears to be normal. (G,H) The sections of a recipient with severe clinical GVH disease injected 50 days earlier with TCD BM and PB CD8⁺ T cells. The colon shows lesions of GVH disease with lymphocytic infiltration of the lamina propria (asterisk) and crypts (black arrows) and apoptotic crypt cells (white arrow). The skin shows hyperplasia (black arrow) and microabscesses (white arrow) in the epidermis and lymphocyte infiltration in the dermis (asterisk). (I,J) The sections of a healthy recipient injected 45 days earlier with TCD BM and BM CD8⁺T cells. No abnormalities are seen. (K,L) The sections of a recipient with slight diarrhea and hair loss injected 45 days earlier with TCD BM and BM NK1.1⁻ CD8⁺ T cells. There are lesions of GVH disease in both tissues, including inflammation of intestine crypts, epidermal hyperplasia, and dermal infiltration.