Different tissue distributions of PB and BM T cells in the allogeneic recipients at 7 days after BM transplantation.

Lethally irradiated BALB/c recipients (H-2K^d) were injected with 1.5 × 10⁶ donor C57BL/6 (H-2K^b, CD45.1) TCD BM and 500 × 10³ PB CD4⁺/CD8⁺ or BM CD4⁺/CD8⁺ T cells from congenic C57BL/6 (H-2K^b, CD45.2) mice. The mononuclear cells from blood, gut, and liver of the recipients were harvested and stained with anti–H-2K^b, TCRαβ, and CD45.2 mAbs. In the first column, panels A, E, and I show the tissues from the recipients given PB CD4⁺/CD8⁺ T cells, and panels C, G, and K show the tissues from the recipients given marrow CD4⁺/CD8⁺ T cells. Analyses in panels A, C, E, G, I, and K show TCRαβ versus H-2K^b with TCRαβ⁺H-2K^b+ cells enclosed in boxes. In the second column, the gated TCRαβ⁺H2k^b+cells were analyzed further for TCRαβ versus CD45.2 in panels B, D, F, H, J, and L. Each panel is representative of 4 recipients.