Effects of monensin and brefeldin A on recovery of CCR5.

CHO.CCR5 cells were pretreated for 1 hour with monensin or brefeldin A or both before internalization was induced. After 30 minutes and 60 minutes, respectively, cells were stained for FACS analysis. (A) MIP-1α. (B) RANTES. (C) MIP-1β. 1, monensin, 30 minutes; 2, monensin, 1 hour; 3, brefeldin A, 30 minutes; 4, brefeldin A, 1 hour; 5, monensin plus brefeldin A, 30 minutes; 6, monensin plus brefeldin A, 1 hour; 7, control, 30 minutes; 8, control, 60 minutes. Data represent mean ± SEM of at least 3 independent experiments.