Fig. 2.
HGF/Met signaling in MM.
(A) HGF stimulation induces Met activation in XG-1 and LME-1. Cells were incubated for 2 minutes in the absence or presence of HGF. Met activation was assessed by immunoprecipitation and subsequent immunoblotting with antiphosphotyrosine antibodies. (B) HGF stimulation induces activation of both PKB/Akt and MAP kinases. Activation of PKB/Akt and MAP kinases was determined in total cell lysates of XG-1, LME-1, and MC cells and immunoblotted with antiphospho PKB/Akt (top) and antiphospho Erk1 and Erk2 (α p-MAPK) (middle), respectively. The Met-expressing Burkitt cell line NamMET was used as a positive control. Stainings with antihuman Met represent loading controls (A,B bottom panels).
