Adhesion of human melanoma cells expressing platelet integrin receptors.

(A) Two transfected human melanoma cell lines expressing integrin receptor αIIbβ3 (▪) or αVβ3 (■) were added to SAA-, fibronectin-, or BSA-coated wells and tested for their adhesion as described. Before addition onto the wells, cells were preincubated (15 minutes at 37°C) with or without Mn²⁺ (2 mM). The results are expressed, after subtracting adhesion to BSA, as a mean ± 1 SEM for 3 experiments. (B) The αIIbβ3 cells were added to SAA-coated wells after preincubation with Mn²⁺ and anti-αIIbβ3 antibody (c7E3Fab) at the indicated concentrations and tested for their adhesion. One representative experiment of 2 performed is shown.