Flow cytometry of human melanoma cells expressing platelet integrin receptors.

(A) Flow cytometry of cells expressing integrin αIIbβ3 (left panel) or αVβ3 (right panel) after incubation with either one of the antibodies, anti-αIIb (clone 5B12), anti-αVβ3 (clone LM609), or control IgG. (B) Flow cytometry of αIIbβ3-expressing cells after incubation with SAA or control BSA followed by staining with a mixture of anti-SAA antibodies (clones mc29 and mc1) or control IgG. (C) Flow cytometry of αIIbβ3- and αVβ3-expressing cells, after incubation with SAA followed by staining with anti-SAA antibodies. Controls for αVβ3 cells were done as described in Figure B (not shown). One representative experiment of 3 performed is shown.