Similar expression of NF-E2, GATA-1, and GpIbα but delay in the GpIIIa expression in MKs derived from CB compared with PB.

(A) RT-PCR analysis of GpIIIa, GpIbα, GATA-1, and NF-E2 mRNA expression during in vitro megakaryocytic differentiation of CD34⁺ cells generated from CB (left panels) and PB (right panels). S26 controls are presented. Representative results from 3 independent experiments are shown. (B) Densitometric analysis (Instant Imager, Packard, Canberra) of GATA-1 and NF-E2 expression normalized to the S26 controls. ●, 1:1, ▪, 1:2; ▴, 1:4 sample dilution. (See also “Materials and methods.”)