Low DNA content of MKs differentiated from CB in comparison with PB-derived CD34⁺ cells.

CD34⁺ cells were cultured for 12 days in serum-free media with TPO (100 ng/mL). MK ploidy was measured by a double-staining technique and flow cytometry (left panels). Histograms of DNA content (right panels) were obtained gating the cells double-labeled with anti-CD61 MoAb and propidium iodide (see also “Materials and methods”). Representative experiment of 5 independent ones is presented.