Lipid raft integrity is necessary for uropod-mediated adhesion and T-cell chemotaxis.

(A) T lymphoblasts were treated or not with CD and, after extensive washing to remove the drug, cell aggregation was induced with anti–ICAM-3 HP2/19 mAb for 30 minutes. Cultures incubated in the absence of mAb or with the control anti–ICAM-3 TP1/24 mAb were analyzed in parallel. A control of anti–ICAM-3 HP2/19 mAb-stimulated cells incubated for 2 hours in the presence of 10% FCS after CD treatment shows that the effect of CD was reversible. The numbers of cell aggregates were quantified in the untreated (white bars) and CD-treated cultures (black bars) and represented as the percentage of aggregates relative to those in the untreated cells stimulated with anti–ICAM-3 HP2/19 mAb. (B) Cells treated or not with CD were induced to migrate toward SDF-1α or RANTES for 2 hours. The numbers of migrated cells in the untreated (white bars) and CD-treated cultures (black bars) were determined and expressed in arbitrary units. The mean ± SD of 4 independent replicates using T lymphoblasts prepared from peripheral blood lymphocytes obtained from different donors is shown in panels A and B.