Purified thrombin activates inflammation-elicited peritoneal macrophages in vivo.

(A) Dose response to thrombin. Four days after TG-stimulated macrophage elicitation (3 mL intraperitoneally), the indicated dosages of purified thrombin were administered, and macrophage numbers in peritoneal exudates were determined after 2.5 hours. (B) Kinetics of macrophage activation in response to thrombin and LPS. Four days after TG-stimulated macrophage elicitation, mice received intraperitoneal injections of thrombin (20 U, ●), LPS (1 μg, ■), or vehicle control (200 μL PBS, ○), and macrophage numbers in peritoneal exudates were determined at the indicated times. For A and B, the data represent the averages and standard deviations of 4 animals per group.