Immunohistochemical study of p14^ARF, Hdm2, and p53 in normal and tumoral lymphoid tissue.

(A) Distinct nucleolar p14^ARF staining, in nontumoral lymphoid tissue (a reactive lymphadenitis). (B) Hdm2 and (C) p53 in the same sample. (D) An NHL case with loss of p14^ARF in tumoral cells (indicated by arrows); normal lymphocytes retain nucleolar p14^ARF expression. Hdm2 (E) and p53 (F) in this case are also shown. (G) An NHL case showing nucleolar p14^ARF in tumoral cells, with intermediate Hdm2 (H) and p53 (I) expression. (J) Nuclear p14^ARF overexpression (note the nucleoli in small lymphocytes) associated with high levels of Hdm2 (K) and p53 overexpression (L). Original magnification × 1000.