American Society of Hematology

Fig. 3.

$Fig. 3. Confocal microscopy analysis of NHL samples. / (A) Double immunolabeling for p14ARF and the nucleolar marker C23 in a sample with p14ARF nuclear overexpression, showing that a significant fraction of the p14ARF signal is extranucleolar. Note the nucleolar p14ARF signal in the normal lymphocyte (right). (B) A sample with intense nucleolar accumulation of p14ARF, coincident with the C23 signal, is shown for comparison. (C) Double immunolabeling for p14ARFand p53 shows a partial colocalization of nucleoplasmic p14ARF and p53. (D) Lack of colocalization in a sample with nucleolar p14ARF, where the discrete granular p14ARF signal contrasts with the nucleoplasmic distribution of p53. (E) Lack of colocalization between nucleoplasmic p14ARF and Hdm2. (F) Hdm2 expression in a tumoral cell where p14ARF is absent; however, nucleolar p14ARF is expressed in adjacent lymphocytes lacking detectable Hdm2. Original magnification × 2000-3500.$

Confocal microscopy analysis of NHL samples.

(A) Double immunolabeling for p14^ARF and the nucleolar marker C23 in a sample with p14^ARF nuclear overexpression, showing that a significant fraction of the p14^ARF signal is extranucleolar. Note the nucleolar p14^ARF signal in the normal lymphocyte (right). (B) A sample with intense nucleolar accumulation of p14^ARF, coincident with the C23 signal, is shown for comparison. (C) Double immunolabeling for p14^ARFand p53 shows a partial colocalization of nucleoplasmic p14^ARF and p53. (D) Lack of colocalization in a sample with nucleolar p14^ARF, where the discrete granular p14^ARF signal contrasts with the nucleoplasmic distribution of p53. (E) Lack of colocalization between nucleoplasmic p14^ARF and Hdm2. (F) Hdm2 expression in a tumoral cell where p14^ARF is absent; however, nucleolar p14^ARF is expressed in adjacent lymphocytes lacking detectable Hdm2. Original magnification × 2000-3500.

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