Representative long-term engraftment analysis of 2 radiation chimeric mice.

This analysis demonstrates high-level multilineage contribution in hematopoietic tissues. CD31⁺c-kit⁺Ly6C⁻ BM-enriched HSCs were cocultured on the UG26-1B6 or the AM14-1C4 stromal clone for 4 weeks and transplanted into irradiated adult recipient mice. At 1 year after transplantation, hematopoietic tissues were obtained and several lineages were sorted. DNA from each population was tested by PCR for the presence of the donor-cell transgenic marker (humanβ-globin). The myogenin (myo) gene served as a control for DNA quantity. Percentage donor contribution to each population is indicated beneath each lane. Contribution controls of 1%, 3%, 10%, 30%, and 100% were made by mixing humanβ-globin transgenic DNA with nontransgenic DNA. Quantitation was performed by densitometry using the myosignal for DNA normalization. PB indicates peripheral blood; B, B lymphocyte; T, T lymphocyte; BM, bone marrow; SPL, spleen; and THY, thymus.