CD25 expression in B-CLL cells treated with ODN.
(A) 1 × 106 purified B-CLL cells were incubated for 48 hours with DSP30, pZ27, or C27 at indicated concentrations. Cells were stained and analyzed by flow cytometry as described in the text. Histograms show fluorescence intensities of the anti-CD25 antibody (shaded), superimposed with that of an isotype control (solid line). Shown is a representative experiment; 2 additional experiments gave similar results. (B) CD25 expression is shown in one sample during a time course of 72 hours in the presence of 1 μM DSP30. Similar results were observed in an independent experiment. (C) Expression of CD25 in B-CLL samples from individual patient’s cells after 48 hours in tissue culture in the presence (black bars) or in the absence (gray bars) of DSP30.
