Analysis of NEPs in bone marrow aspirate.

NEPs were discriminated from other marrow cells by simultaneous labeling of the cell surface with mAb against human glycophorin A and staining of cell DNA with propidium iodide. Three cell populations are identified: (A) peripheral red blood cells (glycophorin A positive only), (B) NEPs (both glycophorin A and DNA positive) and (C) nonerythroid-nucleated marrow cells (DNA positive only).