American Society of Hematology

Fig. 6.

$Fig. 6. Strong anion exchange HPLC fractionation and identification of low-molecular–weight components in plasma used by MPO to initiate lipid peroxidation. / Plasma was filtered through a 10-kd MWt cutoff filter. The filtrate containing the low-molecular–weight components was then fractionated by HPLC using a strong anion exchange column as described in “Materials and methods.” Each fraction was then assessed for its capacity to provide cosubstrate for the MPO–H2O2 system and initiate LDL lipid peroxidation as in Figure 5. Retention times of some compounds described as MPO substrates in vitro include: serotonin, estradiols, Cl−, fraction 3 (F3); tyrosine, F4; ascorbic acid, F5; NO2−, F6; SCN−, F7.$

Strong anion exchange HPLC fractionation and identification of low-molecular–weight components in plasma used by MPO to initiate lipid peroxidation.

Plasma was filtered through a 10-kd MWt cutoff filter. The filtrate containing the low-molecular–weight components was then fractionated by HPLC using a strong anion exchange column as described in “Materials and methods.” Each fraction was then assessed for its capacity to provide cosubstrate for the MPO–H₂O₂ system and initiate LDL lipid peroxidation as in Figure 5. Retention times of some compounds described as MPO substrates in vitro include: serotonin, estradiols, Cl⁻, fraction 3 (F3); tyrosine, F4; ascorbic acid, F5; NO $_{2}^{-}$ ⁠, F6; SCN⁻, F7.

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